Fig 2: P53 deficiency affects bile acid-mediated cell proliferation repression and intestinal metaplasia. (A) HET1A cells with P53 knockdown by shRNA, Western Blot assay of P53 expression level. (B) The proliferation of HET1A cells with or without P53 knockdown after 30 days of continuous exposure to ABS or BS. (C) Representative immunofluorescence of Ki67 images of HET1A cells with 400 µmol/L ABS or BS treatment. (D) Representative immunofluorescence of Ki67 images of P53 knockdown HET1A cells with 400 µmol/L ABS or BS treatment. (E) Quantification of Ki67 expression levels in HET1A cells with or without P53 knockdown stimulated by ABS and BS. (F) After continuous exposure to ABS for denoted time, the expression levels of squamous epithelium marker CK13, columnar epithelium marker CK8 and intestinal metaplasia marker CDX2. (G) After continuous exposure to BS for denoted time, the expression levels of CK13, CK8, and CDX2. +: Treated accordingly; -: Not treated accordingly. Data were statistically analyzed using the student t-test. * p < 0.05, ** p < 0.01, ns: none significance.